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| Reactivity: | M,R & M,R |
| Applications: | IHC & IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Bub1 rabbit polyclonal |
Gene Name: | Mitotic checkpoint serine/threonine-protein kinase BUB1 |
Synonyms: | BUB1, BUB1A, BUB1L, hBUB1 mitotic checkpoint serine/threonine kinase, Budding uninhibited by benzimidazoles 1 homolog |
Immunogen: | Recombinant protein corresponding to Mouse Bub1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Uniprot ID: | O08901 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
IHC | Mouse, Rat | 1: 2000-1: 4000 | spleen, lung, testis |
IF | Mouse, Rat | 1: 1000-1: 2000 | spleen, lung, testis |
Background
Bub1 was initially identified as the mammalian homolog to the yeast protein through a cDNA screen. Bub1 encodes a serine/threonine-protein kinase involved in the formation of the spindle checkpoint, an important mechanism that ensures high fidelity mitotic chromosome segregation. The encoded protein functions in part by phosphorylating members of the mitotic checkpoint complex and activating the spindle checkpoint. This protein also plays a role in inhibiting the activation of the anaphase promoting complex/cyclosome. This protein may also function in the DNA damage response. Mutations in this gene have been associated with aneuploidy and several forms of cancer. Alternate splicing results in multiple transcript variants.
Images
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Immunohistochemistry analysis of paraffin-embedded mouse spleen using Bub1 (GB11979) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat lung using Bub1 (GB11979) at dilution of 1: 2000 |
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Immunohistochemistry analysis of paraffin-embedded rat testis using Bub1 (GB11979) at dilution of 1: 4000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
