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Anti-CAPON/NOS1AP Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | CAPON/NOS1AP rabbit polyclonal |
Gene Name: | Carboxyl-terminal PDZ ligand of neuronal nitric oxide synthase protein |
Synonyms: | C-terminal PDZ ligand of neuronal nitric oxide synthase protein, Nitric oxide synthase 1 adaptor protein, Nos1ap, Capon, Kiaa0464 |
Immunogen: | Recombinant protein corresponding to Mouse CAPON/NOS1AP |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane, Cytoplasm, Nucleus |
Predicted MW. | 56 kDa |
Observed MW. | 56 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | heart, brain |
IHC | Mouse, Rat | 1: 2000-1: 4000 | striatum, heart, hippocampus |
Background
CAPON (carboxy-terminal PDZ ligand of nNOS) selectively binds within the 100 amino acid PDZ domain of the neuronal nitric oxide synthase (nNOS), but not to endothelial NOS or inducible NOS, and sequesters nNOS in the cytosol. Biosynthesis of the neurotransmitter nitric oxide (NO) requires the association of nNOS with various synaptic proteins, including syntrophin, postsynaptic density (PSD)95 and PSD93 through a scaffolding PDZ domain. These proteins facilitate the transport of nNOS to the plasma membrane, where it is catalytically activated by NMDA-receptor mediated calcium channels. The association of nNOS with PSD95 or PSD93 is regulated by CAPON.
Images
| Western blot analysis of NOS1AP (GB113043) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin-embedded mouse striatum using NOS1AP (GB113043) at dilution of 1: 4000 |
| Immunohistochemistry analysis of paraffin-embedded mouse heart using NOS1AP (GB113043) at dilution of 1: 4000 |
| Immunohistochemistry analysis of paraffin-embedded rat heart using NOS1AP (GB113043) at dilution of 1: 4000 |
| Immunohistochemistry analysis of paraffin-embedded rat hippocampus using NOS1AP (GB113043) at dilution of 1: 4000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
