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| Reactivity: | H |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | CD20 rabbit polyclonal antibody |
Gene Name: | B-lymphocyte antigen CD20 |
Synonyms: | MS4A1, B1, Bp35, CD20, CVID5, LEU-16, MS4A2, S7, membrane spanning 4-domains A1 |
Immunogen: | Recombinant protein corresponding to Mouse CD20 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Membrane |
Predicted MW. | 32 KDa |
Observed MW. | 37 KDa |
Uniprot ID: | P19437 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 500-1: 1000 | spleen, lymph gland, thymus |
IHC/IF | Mouse, Rat | 1: 500-1: 3000 | bone, spleen |
Background
B-lymphocyte antigen CD20 or CD20 is an activated-glycosylated phosphoprotein expressed on the surface of all B-cells beginning at the pro-B phase (CD45R+, CD117+) and progressively increasing in concentration until maturity. The protein has no known natural ligand[8] and its function is to enable optimal B-cell immune response, specifically against T-independent antigens. It is suspected that it acts as a calcium channel in the cell membrane. It has been shown that CD20 plays a role in the microenvironmental interactions of B cells.
Images
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Western blot analysis of CD20 (GB11540) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded mouse spleen using CD20 (GB11540) at dilution of 1: 3000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat spleen using CD20 (GB11540) at dilution of 1: 3000 (400x lens) |
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Immunofluorescence of paraffin embedded mouse bone using CD20 (GB11540) at dilution of 1: 500 (400x lens) |
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Immunofluorescence of paraffin embedded mouse spleen using CD20 (GB11540) at dilution of 1: 500 (400x lens) |
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Immunofluorescence of paraffin embedded rat spleen using CD20 (GB11540) at dilution of 1: 500 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
