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| Reactivity: | H & H |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | B-cell receptor CD22 |
Synonyms: | CD22, SIGLEC-2, SIGLEC2, CD22 molecule |
Immunogen: | Recombinant protein corresponding to Human CD22 |
Uniprot ID: | P20273 |
Isotype: | lgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. / Observed MW. | 97 kDa / 140 kDa |
Product Usage Information
WB | Human | 1: 500-1: 1000 | Raji, NALM6 |
IHC/IF | Human | 1: 500-1: 1000 | tonsil, spleen, liver, liver cancer |
Background
CD22 is a molecule belonging to the SIGLEC family of lectins. It is found on the surface of mature B cells and to a lesser extent on some immature B cells. Generally speaking, CD22 is a regulatory molecule that prevents the over-activation of the immune system and the development of autoimmune diseases. CD22 is a sugar binding transmembrane protein, which specifically binds sialic acid with an immunoglobulin (Ig) domain located at its N-terminus.
Images
| WB analysis of CD22 (GB115558). |
| IHC analysis of CD22 (GB115558). Sample: Human tonsil (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of CD22 (GB115558). |
| IHC analysis of CD22 (GB115558). Sample: Human liver (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 600, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of CD22 (GB115558). Sample: Human gastric carcinoma (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of CD22 (GB115558). Sample: Human liver cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 600, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
