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| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 5'-nucleotidase |
Synonyms: | 5'-NT, CD73, Nt5e, Nt5, Nte, 5' nucleotidase precursor, E5NT, Ecto-5'-nucleotidase |
Immunogen: | Recombinant protein corresponding to Mouse CD73 |
Uniprot ID: | Q61503 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. / Observed MW. | 64 kDa / 70 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 300-1: 600 | liver, spleen, placenta |
IHC/IF | Human, Mouse, Rat | 1: 300-1: 600 | lung adenocarcinoma, liver cancer, kindey, colon, liver, placenta |
Background
CD73 is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts.
Images
| Western blot analysis of CD73 (GB115174) at dilution of 1: 600 |
| Immunohistochemistry analysis of paraffin embedded mouse kindey using CD73 (GB115174) at dilution of 1: 300 |
| Immunohistochemistry analysis of paraffin embedded mouse colon using CD73 (GB115174) at dilution of 1: 300 |
| Immunohistochemistry analysis of paraffin embedded human liver cancer using CD73 (GB115174) at dilution of 1: 300 |
| Immunohistochemistry analysis of paraffin embedded rat kindey using CD73 (GB115174) at dilution of 1: 300 |
| Immunohistochemistry analysis of paraffin embedded rat colon using CD73 (GB115174) at dilution of 1: 300 |
| Immunohistochemistry analysis of paraffin embedded human placenta using CD73 (GB115174) at dilution of 1: 300 |
| Immunohistochemistry analysis of paraffin embedded human lung adenocarcinoma using CD73 (GB115174) at dilution of 1: 300 |
| Immunohistochemistry analysis of paraffin embedded rat liver using CD73 (GB115174) at dilution of 1: 300 |
| Immunohistochemistry analysis of paraffin embedded mouse liver using CD73 (GB115174) at dilution of 1: 300 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
