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Anti-CDKN2A/p16INK4a Rabbit pAb (100 μl)
| Reactivity: | H & H |
| Applications: | IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Cyclin-dependent kinase inhibitor 2A |
Synonyms: | Cyclin-dependent kinase 4 inhibitor A, CDK4I, p16-INK4a, Cdkn2a, P16ink4a |
Immunogen: | Recombinant protein corresponding to Mouse CDKN2A |
Uniprot ID: | Q64364 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Images
IHC/IF | Human | 1: 500-1: 2000 | breast cancer, colon, lung cancer |
ICC/IF | Human | 1: 200-1: 800 | HeLa |
Background
This gene generates several transcript variants which differ in their first exons. At least three alternatively spliced variants encoding distinct proteins have been reported, two of which encode structurally related isoforms known to function as inhibitors of CDK4 kinase. The remaining transcript includes an alternate first exon located 20 Kb upstream of the remainder of the gene, this transcript contains an alternate open reading frame (ARF) that specifies a protein which is structurally unrelated to the products of the other variants.
Images
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Immunohistochemistry of paraffin embedded human breast cancer using CDKN2A (GB111143) at dilution of 1:1000 (400x lens) |
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Immunohistochemistry of paraffin embedded human colon using CDKN2A (GB111143) at dilution of 1:1000 (400x lens) |
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Immunohistochemistry of paraffin embedded human lung cancer using CDKN2A (GB111143) at dilution of 1:1000(400x lens) |
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Immunohistochemistry of paraffin embedded human ovarian cancer using CDKN2A (GB111143) at dilution of 1:1000 (400x lens) |
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Immunofluorescence of paraffin embedded human breast cancer using CDKN2A (GB111143) at dilution of 1:500 (400x lens) |
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Immunofluorescence of paraffin embedded human colon using CDKN2A (GB111143) at dilution of 1:500 (400x lens) |
| ICC analysis of CDKN2A(GB111143)red. Sample: HeLa, 4% PFA (G1101) 20min. Primary antibody: 1: 400, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
