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| Reactivity: | M,R & M,R & M |
| Applications: | WB & IHC/IF & ICC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Cytochrome c oxidase subunit 4 isoform 1, mitochondrial |
Synonyms: | Cytochrome c oxidase polypeptide IV, Cytochrome c oxidase subunit IV isoform 1, COX IV-1, Cox4i1, Cox4, Cox4a, COX4I1, COX4, COX4-1, COXIV, COXIV-1, COXIV-1, cytochrome c oxidase subunit 4I1 |
Immunogen: | Recombinant protein corresponding to Mouse COX IV |
Uniprot ID: | P19783 |
Isotype: | IgG2a |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion inner membrane |
Predicted MW. / Observed MW. | 20 kDa / 17 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 1000-1: 2000 | heart, liver, brain |
IHC/IF | Mouse, Rat | 1: 1000-1: 2000 | brain, heart, kidney |
ICC/IF | Mouse | 1:50-1:200 | NIH-3T3 |
Background
COX4I1, also named as COX4 and COXIV-1, belongs to the cytochrome c oxidase IV family. It is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. COX4I1 is a marker for mitochondria. It has two isoforms (isoform 1 and 2). Isoform 1(COX4I1) is ubiquitously expressed and isoform 2 is highly expressed in lung tissues. COX4I1 is commonly used as a loading control. This antibody was generated against full length COX4I1 protein and cross reacts with COX4I2
Images
| Western blot analysis of COX4 (GB12250) at dilution of 1: 1000 |
| Immunohistochemistry of paraffin embedded mouse brain using COX4 (GB12250) at dilution of 1: 1000 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse heart using COX4 (GB12250) at dilution of 1: 1000 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse kidney using COX4 (GB12250) at dilution of 1: 1000 (400x lens) |
| Immunohistochemistry of paraffin embedded rat heart using COX4 (GB12250) at dilution of 1: 1000 (400x lens) |
| Immunohistochemistry of paraffin embedded rat kidney using COX4 (GB12250) at dilution of 1: 1000 (400x lens) |
| Immunohistochemistry of paraffin embedded rat liver using COX4 (GB12250) at dilution of 1: 1000 (400x lens) |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using COX IV(GB12250) at dilution of 1:50-1:200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
