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| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Full Name: | CREB mouse monoclonal |
Gene Name: | Cyclic AMP-responsive element-binding protein 1 |
Synonyms: | CREB1, CREB, CREB-1, cAMP responsive element binding protein 1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human CREB-1. |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 37 kDa |
Observed MW. | 37 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | spleen, lung, brain |
Background
Cyclic AMP Response Element Binding protein (CREB) is a basic / leucine zipper transcription factor that binds the cyclic AMP response element (CRE) and activates transcription in response to a variety of extracellular signals including neurotransmitters, hormones, membrane depolarization, and growth and neurotrophic factors. Activation of CREB is dependent upon the phosphorylation of serine 133. Phosphorylation occurs via p44 / 42 MAP kinase and p90RSK and also via p38 MAP kinase and MSK 1. Although CREB will bind DNA independent of its phosphorylation state, only the phosphorylated form is competent as a transcription factor. CREB binding protein (CBP), a transcriptional coactivator that directly interacts with CREB, binds to CREB in the region of serine 133. CREB appears to play an important role in learning and memory. CREB knock out mice show diminished learning ability.
Images
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Western blot analysis of CREB (GB13236-1) at dilution of 1: 1000. Lane 1: HeLa cell lysate Lane 2: HepG2 cell lysate Lane 3: NIH3T3 cell lysate Lane 4: Mouse spleen tissue lysate Lane 5: Mouse lung tissue lysate Lane 6: Mouse brain tissue lysate Lane 7: Rat spleen tissue lysate Lane 8: Rat lung tissue lysate Lane 9: Rat brain tissue lysate |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
