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| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | CTCF rabbit polyclonal antibody |
Gene Name: | Transcriptional repressor CTCF |
Synonyms: | 11-zinc finger protein, CCCTC-binding factor, CTCFL paralog, Ctcf |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse CTCF |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Uniprot ID: | Q61164, Q9R1D1 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
IHC/IF | Mouse, Rat | 1: 500-1: 2000 | brain, colon, heart, lung, skin, small intestine, spleen |
Background
Transcriptional factor CTCF is a ubiquitously expressed 11-zinc finger domain containing, DNA-binding nuclear phosphoprotein that is involved in multiple aspects of normal gene regulation including transcriptional repression and activation, gene silencing, chromatin insulation and regulation of imprinted sites. CTCF uses different combinations of ZF domains and interacts with the CCCTC motif on the DNA. It can bind to HAT- and HDAC-containing complexes thereby influencing transcriptional activation or repression respectively.
Images
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Immunohistochemistry of paraffin embedded mouse brain using CTCF (GB111225) at dilution of 1:1000 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse colon using CTCF (GB111225) at dilution of 1:1000 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse heart using CTCF (GB111225) at dilution of 1:1000 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse lung using CTCF (GB111225) at dilution of 1:1000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat skin using CTCF (GB111225) at dilution of 1:1000 (400x lens) |
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Immunohistochemistry of paraffin embedded rat small intestine using CTCF (GB111225) at dilution of 1:1000 (400x lens) |
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Immunofluorescence of paraffin embedded mouse brain using CTCF (GB111225) at dilution of 1:500 (400x lens) |
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Immunofluorescence of paraffin embedded rat brain using CTCF (GB111225) at dilution of 1:500 (400x lens) |
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Immunofluorescence of paraffin embedded rat spleen using CTCF (GB111225) at dilution of 1:500 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
