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Anti-CYP51A1/CYP51 Rabbit pAb (100 μl)
| Reactivity: | H & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Lanosterol 14-alpha demethylase |
Synonyms: | CP51, CYP51, CYP51A1, CYPL1, CYPLI, Cytochrome P450 14DM, Cytochrome P450 51A1, Cytochrome P45014DM, Cytochrome P450LI, LDM, P450 14DM, P450L1, Sterol 14 alpha demethylase |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse CYP51A1/CYP51 |
Uniprot ID: | Q8K0C4 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Endoplasmic reticulum membrane, Microsome membrane |
Predicted MW. / Observed MW. | 57 kDa / 60 kDa |
Product Usage Information
WB | Human | 1: 800-1: 1500 | HeLa, A-431, MCF7 |
IHC/IF | Mouse, Rat | 1: 800-1: 2400 | colon, kidney |
Background
A cytochrome P450 monooxygenase involved in sterol biosynthesis. Catalyzes 14-alpha demethylation of lanosterol and 24,25-dihydrolanosterol likely through sequential oxidative conversion of 14-alpha methyl group to hydroxymethyl, then to carboxylaldehyde, followed by the formation of the delta 14,15 double bond in the sterol core and concomitant release of formic acid. Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate, and reducing the second into a water molecule, with two electrons provided by NADPH via cytochrome P450 reductase (CPR; NADPH-ferrihemoprotein reductase).
Images
| Western blot analysis of CYP51A1 (GB114898) at dilution of 1: 800 |
| Immunohistochemistry of paraffin embedded rat colon using CYP51A1 (GB114898) at dilution of 1: 800 |
| Immunohistochemistry of paraffin embedded rat kidney using CYP51A1 (GB114898) at dilution of 1: 800 |
| Immunohistochemistry of paraffin embedded mouse kidney using CYP51A1 (GB114898) at dilution of 1: 800 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
