Recombinant Anti-Carbonic Anhydrase 9/CA9 Mouse mAb (100 μl)

Brand: ServiceBio
Cat. #: GB151184-100
Availability: In Stock

$259.00

Qty

Reactivity:	H
Applications:	IHC/IF
Host Species:	Mouse
Clonality:	Monoclonal
Gene Name:	Carbonic anhydrase 9
Synonyms:	CA IX, CA9, CAIX, Carbonate dehydratase IX, Carbonic anhydrase 9, carbonic anhydrase IX, G250, Membrane antigen MN, MN, P54/58N, pMW1, RCC associated antigen G250
Immunogen:	Recombinant protein corresponding to Human CA9
Uniprot ID:	Q16790
Isotype:	lgG1
Purity:	Affinity purification
Subcellular location:	Nucleus, Cell membrane, Cell projection
Predicted MW. / Observed MW.	47 kDa / 50 kDa

Product Usage Information

IHC/IF

Human

1: 500-1: 1000

stomach, colon cancer

Background

Catalyzes the interconversion between carbon dioxide and water and the dissociated ions of carbonic acid (i.e. bicarbonate and hydrogen ions).

Images

	IHC analysis of Carbonic Anhydrase (GB151184). Sample: Human stomach (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h.
	IHC analysis of Carbonic Anhydrase (GB151184). Sample: Human colon cancer (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h.

Storage

Storage	Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer	PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.