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Anti-Cdc25A Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,R,M |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Cdc25A rabbit polyclonal |
Gene Name: | M-phase inducer phosphatase 1 |
Synonyms: | CDC25A, CDC25A2, cell division cycle 25A,Dual Specificity Phosphatase CDC25A |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Cdc25A |
Isotype: | IgG |
Predicted MW. | 59 kDa |
Observed MW. | 70 kDa |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | spleen, colon, stomach |
IHC | Human, Mouse, Rat | 1: 1500-1: 3000 | colon, tumor, brain |
IF | Human, Mouse, Rat | 1: 1500-1: 3000 | colon, tumor, brain |
Background
CDC25A is a member of the CDC25 family of phosphatases. CDC25A is required for progression from G1 to the S phase of the cell cycle. It activates the cyclin-dependent kinase CDC2 by removing two phosphate groups. CDC25A is specifically degraded in response to DNA damage, which prevents cells with chromosomal abnormalities from progressing through cell division. CDC25A is an oncogene, although its exact role in oncogenesis has not been demonstrated. Two transcript variants encoding different isoforms have been found for this gene.
Images
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Western blot analysis of Cdc25A(GB11283) at dilution of 1:1000. Lane1:HeLa cell lysate Lane2:HepG2 cell lysate Lane3:Jurkat cell lysate Lane 4:Mouse spleen tissue lysate Lane 5:Mouse stomach tissue lysate Lane 6:Mouse colon tissue lysate Lane 7:Rat spleen tissue lysate Lane 8:Rat stomach tissue lysate Lane 9:Rat colon tissue lysate |
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Immunohistochemistry analysis of paraffin-embedded human colon using Cdc25A(GB11283) at dilution of 1:3000 |
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Immunohistochemistry analysis of paraffin-embedded mouse tumor using Cdc25A(GB11283) at dilution of 1:3000 |
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Immunohistochemistry analysis of paraffin-embedded rat brain using Cdc25A(GB11283) at dilution of 1:3000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
