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Anti-Collagen I Rabbit pAb (100 μl)
| Reactivity: | M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Collagen alpha-1(I) chain |
Synonyms: | COL1A1, EDSC, OI1, OI2, OI3, OI4, collagen type I alpha 1, collagen type I alpha 1 chain |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human Collagen I |
Uniprot ID: | P02452 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Extracellular matrix |
Predicted MW. / Observed MW. | 139kDa / 120-140 kDa/200-250 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Mouse, Rat | 1: 500-1: 1000 | skin |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1500 | lung, endometrial carcinoma |
Background
This gene encodes the pro-alpha1 chains of type I collagen whose triple helix comprises two alpha1 chains and one alpha2 chain. Type I is a fibril-forming collagen found in most connective tissues and is abundant in bone, cornea, dermis and tendon. Mutations in this gene are associated with osteogenesis imperfecta types I-IV, Ehlers-Danlos syndrome type VIIA, Ehlers-Danlos syndrome Classical type, Caffey Disease and idiopathic osteoporosis.
Images
| WB analysis of Collagen I(GB115707). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| IHC analysis of Collagen I(GB115707). Sample: Mouse lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
| IHC analysis of Collagen I(GB115707). Sample: Human colon cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
| IHC analysis of Collagen I(GB115707). Sample: Human endometrial carcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
| IHC analysis of Collagen I(GB115707). Sample: Human esophageal carcinoma, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
| IHC analysis of Collagen I(GB115707). Sample: Mouse fibrotic liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
| IHC analysis of Collagen I(GB115707). Sample: Mouse skin, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
| IHC analysis of Collagen I(GB115707). Sample: Rat fibrotic kidney, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
| IHC analysis of Collagen I(GB115707). Sample: Rat lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
| IHC analysis of Collagen I(GB115707). Sample: Rat skin, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C, 20 min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
