Anti-Collagen II Mouse mAb (100 μl)

Collagen alpha-1(II) chain

Synonyms:

Collagen alpha-1(II) chain

Immunogen:

Recombinant protein corresponding to Mouse collagen II

Uniprot ID:

P28481

Isotype:

IgG2a,κ

Purity:

Affinity purification

Subcellular location:

Secreted

IHC/IF

Mouse, Rat

1: 700-1: 2500

bone

IHC analysis of Collagen II (GB12021).
Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 2600, 4°C overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.

IHC analysis of Collagen II (GB12021).
Sample: Mouse bone (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 2600, 4°C overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.

IHC analysis of Collagen II (GB12021).
Sample: Rat bone (Paraffin), 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201),98°C,20 min.
Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min.
Primary antibody: 1: 2600, 4°C overnight.
Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 200 RT 1h.