Recombinant Anti-Collagen II Rabbit mAb (100 μl)

Collagen alpha-1(II) chain

Synonyms:

COL2A1, ANFH, AOM, COL11A3, SEDC, STL1, collagen type II alpha 1, collagen type II alpha 1 chain

Immunogen:

Recombinant protein corresponding to Mouse collagen II

Uniprot ID:

P28481

Isotype:

IgG

Purity:

Affinity purification

Subcellular location:

Secreted, Extracellular matrix

Applications

Species

Dilution

Positive Tissue

IHC/IF

Mouse, Rat

1: 500-1: 1000

cartilage

IHC analysis of Collagen II(GB150010).
Sample: Mouse cartilage, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 95°C, 20 min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min.

IHC analysis of Collagen II(GB150010).
Sample: Rat cartilage, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 95°C, 20 min.
Primary antibody: 1: 500, 4°C overnight.
Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Rabbit lgG(H+L),Ready to use(G1302), RT, 20min.