Anti-Collagen III Rabbit pAb (100 μl)

Collagen alpha-1(III) chain

Synonyms:

Col3a1, EDS4A, type III preprocollagen alpha 1 chain, Type III collagen, Collagen III alpha 1 chain precursor , Collagen III alpha 1 polypeptide

Immunogen:

Recombinant protein corresponding to Human Collagen III

Uniprot ID:

P02461

Isotype:

IgG

Purity:

Affinity purification

Subcellular location:

Secreted

Predicted MW. / Observed MW.

138 kDa / 140-220 kDa

WB

Human

1: 300-1: 500

HeLa, HT-1080, HS578T, MCF7

IHC/IF

Human

1: 200-1: 400

esophageal carcinoma, kidney

WB analysis of Collagen III(GB115674).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 300, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h.

IHC analysis of Collagen III(GB115674).
Sample: Human esophageal carcinoma, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min.
Primary antibody: 1: 200, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.

IHC analysis of Collagen III(GB115674).
Sample: Human kidney, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), 98°C 20min.
Primary antibody: 1: 200, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 200, RT, 1h.