Your shopping cart is empty!
Recombinant Anti-Cyclin A2 Mouse mAb (100 μl)
| Reactivity: | H |
| Applications: | WB |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Cyclin-A2 |
Synonyms: | Cyclin-A, Ccna2, CycA, CycA2, Cyclin A2,Cyclin A, CCNA2, CCNA, CCN1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse Cyclin A2 |
Uniprot ID: | P51943 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 49 kDa / 55 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Human | 1: 500-1: 1000 | HT-29, A549, K562 |
Background
Cyclin A2 belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance through the cell cycle. Cyclins function as regulators of CDK kinases. Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. In contrast to cyclin A1, which is present only in germ cells, this cyclin is expressed in all tissues tested. Cyclin A2 binds and activates CDC2 or CDK2 kinases, and thus promotes both cell cycle G1/S and G2/M transitions. Cyclin A2 could be phosphorylated during the G2/M.
Images
| WB analysis of Cyclin A2(GB151228). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
