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Anti-DDX19B Rabbit pAb (100 μl)
| Reactivity: | H,M & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | DDX19B rabbit polyclonal antibody |
Gene Name: | ATP-dependent RNA helicase DDX19B |
Synonyms: | DEAD box RNA helicase DEAD5, DEAD box protein 19B, DDX19B, DBP5, DDX19, TDBP |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human DDX19B |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. | 54 kDa |
Observed MW. | 70 kDa |
Uniprot ID: | Q9UMR2 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse | 1: 800-1: 1600 | HeLa, HepG2, 293T, A549, HEK293, K562, NIH3T3 |
IHC/IF | Human, Mouse, Rat | 1: 600-1: 3000 | brain, breast, breast cancer, gastric cancer, heart, spinal cord, stomach |
Background
DEAD box proteins, characterized by the conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases. They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. This gene encodes a DEAD box protein, which exhibits RNA-dependent ATPase and ATP-dependent RNA-unwinding activities. This protein is recruited to the cytoplasmic fibrils of the nuclear pore complex, where it participates in the export of mRNA from the nucleus.
Images
| Western blot analysis of DDX19B (GB112551) at dilution of 1: 800 |
| Immunohistochemistry of paraffin embedded human gastric cancer using DDX19B (GB112551) at dilution of 1: 1000 (400x lens) |
| Immunohistochemistry of paraffin embedded human stomach using DDX19B (GB112551) at dilution of 1: 1000 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse heart using DDX19B (GB112551) at dilution of 1: 1000 (400x lens) |
| Immunohistochemistry of paraffin embedded mouse spinal cord using DDX19B (GB112551) at dilution of 1: 1000 (400x lens) |
 | Immunohistochemistry of paraffin embedded rat brain using DDX19B (GB112551) at dilution of 1: 1000 (400x lens) |
| Immunohistochemistry of paraffin embedded rat heart using DDX19B (GB112551) at dilution of 1: 1000 (400x lens) |
| Immunofluorescence of paraffin embedded human breast cancer using DDX19B (GB112551) at dilution of 1: 650 (400x lens) |
| Immunofluorescence of paraffin embedded mouse breast using DDX19B (GB112551) at dilution of 1: 650 (400x lens) |
| Immunofluorescence of paraffin embedded rat breast using DDX19B (GB112551) at dilution of 1: 650 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |