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Anti-EEF2/Elongation factor 2 Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Elongation factor 2 |
Synonyms: | EF-2, Eef2, Eukaryotic translation elongation factor 2; Eukaryotic Elongation Factor-2 |
Immunogen: | Recombinant protein corresponding to Mouse EEF2 |
Uniprot ID: | P58252 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. / Observed MW. | 95 kDa / 95 kDa |
Images
WB | Human, Mouse, Rat | 1: 500-1: 1000 | pancreas, liver, ovary |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 4000 | spleen, lung, colon |
Background
This gene encodes a member of the GTP-binding translation elongation factor family. This protein catalyzes the GTP-dependent ribosomal translocation step during translation elongation. During this step, the ribosome changes from the pre-translocational (PRE) to the post- translocational (POST) state as the newly formed A-site-bound peptidyl-tRNA and P-site-bound deacylated tRNA move to the P and E sites, respectively. Catalyzes the coordinated movement of the two tRNA molecules, the mRNA and conformational changes in the ribosome.
Images
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Western blot analysis of ERC2 (GB111055) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin embedded rat thymus using eef2 (GB111099) at dilution of 1: 4000 |
| Immunohistochemistry analysis of paraffin embedded rat spleen using eef2 (GB111099) at dilution of 1: 2000 |
| Immunohistochemistry analysis of paraffin embedded rat ovary using eef2 (GB111099) at dilution of 1: 4000 |
| Immunohistochemistry analysis of paraffin embedded mouse thymus using eef2 (GB111099) at dilution of 1: 2000 |
| Immunohistochemistry analysis of paraffin embedded mouse spleen using eef2 (GB111099) at dilution of 1: 2000 |
| Immunohistochemistry analysis of paraffin embedded mouse lung using eef2 (GB111099) at dilution of 1: 4000 |
| Immunofluorescent analysis of paraffin embedded rat thymus using eef2 (GB111099) at dilution of 1: 3000 |
| Immunofluorescent analysis of paraffin embedded rat liver using eef2 (GB111099) at dilution of 1: 3000 |
| Immunofluorescent analysis of paraffin embedded mouse spleen using eef2 (GB111099) at dilution of 1: 3000 |
| Immunofluorescent analysis of paraffin embedded mouse ovary using eef2 (GB111099) at dilution of 1: 3000 |
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Immunofluorescent analysis of paraffin embedded human colon using eef2 (GB111099) at dilution of 1: 3000 |
| Immunofluorescent analysis of paraffin embedded human colon using eef2 (GB111099) at dilution of 1: 3000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
