Reactivity: | H & H |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | Forkhead box protein O1 |
Synonyms: | FOXO1, FKH1, FKHR, FOXO1A, forkhead box O1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human Foxo1 |
Uniprot ID: | Q12778 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. / Observed MW. | 69 kDa / 69 kDa |
Product Usage Information
WB | Human | 1: 500-1: 1000 | HeLa, 293T, MCF7, Hep G2, LO2 |
IHC/IF | Human | 1: 700-1: 1400 | renal carcinoma, gastric carcinoma, colon cancer |
Background
FOXO1 is a transcription factor that plays important roles in regulation ofgluconeogenesis and glycogenolysis by insulin signaling, and is also central to the decision for a preadipocyte to commit to adipogenesis. It is primarily regulated through phosphorylation on multiple residues; its transcriptional activity is dependent on its phosphorylation state.
Images
Western blot analysis of C2077 (GB114935) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin embedded human renal carcinoma using FOXO1 (GB114935) at dilution of 1: 700 | |
Immunohistochemistry analysis of paraffin embedded human gastric carcinoma using FOXO1 (GB114935) at dilution of 1: 700 | |
Immunohistochemistry analysis of paraffin embedded human colon cancer using FOXO1 (GB114935) at dilution of 1: 1000 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |