Recombinant Anti-Fatty Acid Synthase Mouse mAb (100 μl)

Recombinant Anti-Fatty Acid Synthase Mouse mAb (100 μl)

Brand: ServiceBio
Cat. #: GB15546-100
Availability: In Stock
$259.00
-+
Reactivity: H,M & M    
Applications: WB & IHC/IF    
Host Species: Mouse    
Clonality: Monoclonal    
Gene Name:

Fatty acid synthase

Synonyms:

FASN, fatty acid synthase, Fasn,A630082H08Rik, FAS, OA-519, SDR27X1,Fatty acid synthase

Immunogen:

Recombinant protein corresponding to Mouse Fatty acid synthase

Uniprot ID:

P19096

Isotype:

IgG1

Purity:

Affinity purification

Subcellular location:

Cytoplasm

Predicted MW. / Observed MW.

273 kDa / 250-273 kDa




Product Usage Information

Applications

Species

Dilution

Positive Tissue

WB

Human, Mouse

1: 500-1: 1000

Hela, MCF7,K532

IHC/IF

Mouse

1: 1000-1: 2000

lung




Background

Fatty acid synthase is a multi-enzyme protein that catalyzes fatty acid synthesis. It is not a single enzyme but a whole enzymatic system composed of two identical 272 kDa multifunctional polypeptides, in which substrates are handed from one functional domain to the next. Its main function is to catalyze the synthesis of palmitate (C16:0, a long-chain saturated fatty acid) from acetyl-CoA and malonyl-CoA, in the presence of NADPH.




Images

01 GB15546_Fatty Acid Synthase_重组 H411_d1000_WB_WM.png

WB analysis of Fatty Acid Synthase(GB15546).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h.

02 GB15546_Fatty Acid Synthase_ZSM18047_ZSM18047231212_d1000_M lung_40.0x_IHC_WM.png

IHC analysis of Fatty Acid Synthase(GB15546).
Sample: Mouse lung, 4% PFA (G1101) 12-24h.
Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h.




Storage

Storage             Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer             PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol.


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