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Anti-Fibronectin Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Fibronectin |
Synonyms: | FN1, CIG, ED-B, FINC, FN, FNZ, GFND, GFND2, LETS, MSF, fibronectin 1 |
Immunogen: | Recombinant protein corresponding to Mouse Fibronectin |
Uniprot ID: | P11276 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Extracellular matrix |
Predicted MW. / Observed MW. | 272 kDa / 300 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 300-1: 600 | serum |
IHC/IF | Human, Mouse, Rat | 1: 200-1: 400 | kidney |
Background
Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.
Anastellin binds fibronectin and induces fibril formation.
Images
 | WB analysis of FN (GB114057). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
 | IHC analysis of FN (GB114057). Sample: Rat kindey (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Proteinase K(GC305007) 200ug/ml,37°C,30minBlocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 900, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
