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Recombinant Anti-Flag Tag Mouse mAb (100 μl)
| Reactivity: | |
| Applications: | WB & ICC/IF & IP |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Flag Tag |
Synonyms: | DDDDK tag, DYKDDDDK, ddk, DDDDK epitope tag, ECS epitope tag, Enterokinase Cleavage Site tag, FLAG tag, FLAG |
Immunogen: | KLH conjugated Synthetic peptide corresponding to DYKDDDDK |
Isotype: | IgG |
Purity: | Affinity purification |
Product Usage Information
WB | Species independent | 1: 500-1: 2000 | Flag-Tag fusion protein |
ICC/IF | Species independent | 1: 500-1: 1000 | Transfected HEK-293T cells |
IP | Species independent | 1: 50-1: 200 | transfected HEK-293F cells |
Special note
Epitope labeling has been proved to be an effective method that can be performed on target proteins. Tag antibody can be used in a variety of applications, including ChIP, CoIP, ELISA, FC, IF, IHC, IP, WB and so on.
Background
The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on.
Images
 | Western blot analysis of FLAG Tag (GB15938) at dilution of 1: 2000 |
| Immunocytochemistry analysis of 4% paraformaldehyde fixed transfected HEK-293T cells using FLAG Tag (GB15938) at dilution of 1: 600 |
| Immunoprecipitation of transfected HEK-293F cells lysate using Flag-Tag（IP: GB15938, 1: 100; Detection: GB11938, 1: 3000） |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
