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Anti-Frizzled 5 Rabbit pAb (100 μl)
| Reactivity: | M,R & H |
| Applications: | WB & IHC |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | Frizzled 5 rabbit polyclonal |
Gene Name: | Frizzled-5 |
Synonyms: | FZD5, C2orf31, HFZ5, frizzled class receptor 5 |
Immunogen: | Recombinant protein corresponding to Mouse Frizzled 5 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 64 kDa |
Observed MW. | 50 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Mouse, Rat | 1: 500-1: 1000 | epencephalon, brain, cortex, small intestine |
IHC | Human | 1: 800-1: 1600 | ovarian cancer |
Background
Receptor for Wnt proteins. Most of frizzled receptors are coupled to the beta-catenin canonical signaling pathway, which leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. A second signaling pathway involving PKC and calcium fluxes has been seen for some family members, but it is not yet clear if it represents a distinct pathway or if it can be integrated in the canonical pathway, as PKC seems to be required for Wnt-mediated inactivation of GSK-3 kinase. Both pathways seem to involve interactions with G-proteins. May be involved in transduction and intercellular transmission of polarity information during tissue morphogenesis and/or in differentiated tissues. Interacts specifically with Wnt5A to induce the beta-catenin pathway.
Images
| Western blot analysis of Frizzled (GB11622) at dilution of 1: 1000 |
|
Immunohistochemistry analysis of paraffin-embedded human ovarian cancer using Frizzled (GB11622) at dilution of 1: 1600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
