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| Reactivity: | M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | GFI1 rabbit polyclonal antibody |
Gene Name: | Zinc finger protein Gfi-1 |
Synonyms: | Growth factor independent protein 1, Zinc finger protein 163, GFI1, ZNF163 |
Immunogen: | Recombinant protein corresponding to Human GFI1 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 45 kDa |
Observed MW. | 50 kDa |
Uniprot ID: | P70338, Q07120 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Mouse, Rat | 1: 1000-1: 2000 | liver, kidney, lung |
Background
Growth factor independent 1 (Gfi-1) is a transcriptional repressor that specifically binds to the DNA consensus sequence TAAATCAC(A/T)GCA. The carboxy-terminus of Gfi-1 contains six C2H2-type zinc finger motifs, and zinc fingers 3, 4 and 5 are required for the binding of Gfi-1 to its DNA binding site. Gfi-1 also contains a 20 amino acid SNAG domain which mediates transcriptional repression. It represses Bax at the mRNA and protein levels, resulting in the inhibition of cell death. Gfi1 is expressed outside the lymphoid system in granulocytes and activated macrophages.
Images
 | Western blot analysis of GFI1 (GB112678) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
