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Anti-GRP78 BiP Rabbit pAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Endoplasmic reticulum chaperone BiP |
Synonyms: | HSPA5, BIP, GRP78, HEL-S-89n, MIF2, Binding immunoglobulin protein, heat shock protein family A (Hsp70) member 5 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse GRP78 |
Uniprot ID: | P20029 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Endoplasmic reticulum lumen, Melanosome, Cytoplasm |
Predicted MW. / Observed MW. | 78 kDa / 78 kDa |
reactivity （External validation） | Pelodiscus sinensis |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | lung, kidney, liver |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | stomach cancer, brain |
Background
Probably plays a role in facilitating the assembly of multimeric protein complexes inside the endoplasmic reticulum. Involved in the correct folding of proteins and degradation of misfolded proteins via its interaction with DNAJC10, probably to facilitate the release of DNAJC10 from its substrate.
Images
| WB analysis of GRP78 (GB11098). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h. |
| IF analysis of GRP78 (GB11098). Sample: Mouse cerebellum (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
| IF analysis of GRP78 (GB11098). Sample: Rat hippocampus (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH 9.0) (G1203),98°C,20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300 RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
