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Anti-Gemin-4 Rabbit pAb (100 μl)
| Reactivity: | M,R & H |
| Applications: | IHC/IF & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Gem-associated protein 4 |
Synonyms: | GEMIN4, HC56, HCAP1, HHRF 1, p97 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human Gemin-4 |
Uniprot ID: | P57678 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Product Usage Information
IHC/IF | Mouse, Rat | 1: 800-1: 2400 | spleen, testis |
ICC/IF | Human | 1: 100-1: 400 | HeLa |
Background
The SMN complex catalyzes the assembly of small nuclear ribonucleoproteins (snRNPs), the building blocks of the spliceosome, and thereby plays an important role in the splicing of cellular pre-mRNAs. Most spliceosomal snRNPs contain a common set of Sm proteins SNRPB, SNRPD1, SNRPD2, SNRPD3, SNRPE, SNRPF and SNRPG that assemble in a heptameric protein ring on the Sm site of the small nuclear RNA to form the core snRNP (Sm core). In the cytosol, the Sm proteins SNRPD1, SNRPD2, SNRPE, SNRPF and SNRPG are trapped in an inactive 6S pICln-Sm complex by the chaperone CLNS1A that controls the assembly of the core snRNP. To assemble core snRNPs, the SMN complex accepts the trapped 5Sm proteins from CLNS1A forming an intermediate. Binding of snRNA inside 5Sm triggers eviction of the SMN complex, thereby allowing binding of SNRPD3 and SNRPB to complete assembly of the core snRNP.
Images
| Immunohistochemistry analysis of paraffin embedded mouse spleen using Gemin4 (GB114982) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin embedded rat testis using Gemin4 (GB114982) at dilution of 1: 800 |
| Immunohistochemistry analysis of paraffin embedded mouse testis using Gemin4 (GB114982) at dilution of 1: 800 |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using Gemin4 (GB114982) at dilution of 1: 200 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
