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Anti-Granzyme B Mouse mAb (100 μl)
| Reactivity: | H |
| Applications: | IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Granzyme B |
Synonyms: | CCPI, CGL 1, CGL1, CSP B, CSPB, CTLA1, CTSGL1, Endogenous granzyme B, Granzyme B, GZMB, HLP, SECT |
Immunogen: | Human recombinant Granzyme B protein expressed in HEK293 cells |
Uniprot ID: | P10144 |
Isotype: | lgG1 |
Purity: | Affinity purification |
Subcellular location: | Cytoplasmic granule |
Product Usage Information
IHC/IF | Human | 1: 500-1: 1500 | colon, tonsil, esophageal cancer |
Background
Abundant protease in the cytosolic granules of cytotoxic T-cells and NK-cells which activates caspase-independent pyroptosis when delivered into the target cell through the immunological synapse. It cleaves after Asp. Once delivered into the target cell, acts by catalyzing cleavage of gasdermin-E (GSDME), releasing the pore-forming moiety of GSDME, thereby triggering pyroptosis and target cell death. Seems to be linked to an activation cascade of caspases (aspartate-specific cysteine proteases) responsible for apoptosis execution. Cleaves caspase-3, -7, -9 and 10 to give rise to active enzymes mediating apoptosis.
Images
| Immunohistochemistry analysis of paraffin embedded human esophageal cancer using GranzymeB (GB12092) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human colon using GranzymeB (GB12092) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded human tonsil using GranzymeB (GB12092) at dilution of 1: 1000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
