Reactivity: | H,M,R & H,M,R & H,M,R |
Applications: | WB & IHC/IF & ICC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | Stress-70 protein, mitochondrial |
Synonyms: | HSPA9, CSA, GRP-75, GRP75, HEL-S-124m, HSPA9B, MOT, MOT2, MTHSP75, PBP74, CRP40, EVPLS, SAAN, SIDBA4, Heat shock protein family A (Hsp70) member 9 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse GRP75 |
Uniprot ID: | P38647 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cytoplasm, Nucleus |
Predicted MW. / Observed MW. | 73 kDa / 73 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | liver, brain, thymus |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 3000 | colon cancer, colon, breast cancer |
Background
This gene encodes a member of the heat shock protein 70 gene family. The encoded protein is primarily localized to the mitochondria but is also found in the endoplasmic reticulum, plasma membrane and cytoplasmic vesicles. This protein is a heat-shock cognate protein. This protein plays a role in cell proliferation, stress response and maintenance of the mitochondria. A pseudogene of this gene is found on chromosome 2.
Images
Western blot analysis of GRP75 (GB11852) at dilution of 1: 1000 | |
Immunohistochemistry analysis of paraffin embedded rat testis using GRP75 (GB11852) at dilution of 1: 3000 | |
Immunohistochemistry analysis of paraffin embedded rat kidney using GRP75 (GB11852) at dilution of 1: 3000 | |
Immunohistochemistry analysis of paraffin embedded mouse jejunum using GRP75 (GB11852) at dilution of 1: 3000 | |
Immunohistochemistry analysis of paraffin embedded human colon using GRP75 (GB11852) at dilution of 1: 3000 | |
Immunohistochemistry analysis of paraffin embedded human colon cancer using GRP75 (GB11852) at dilution of 1: 3000 | |
Immunofluorescent analysis of paraffin embedded rat testis using GRP75 (GB11852) at dilution of 1: 1000 | |
Immunofluorescent analysis of paraffin embedded mouse kidney using GRP75 (GB11852) at dilution of 1: 1000 | |
Immunofluorescent analysis of paraffin embedded human breast cancer using GRP75 (GB11852) at dilution of 1: 1000 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |