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Anti-H2ab1 Rabbit pAb (100 μl)
| Reactivity: | M & M |
| Applications: | WB & IHC |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | H2ab1 rabbit polyclonal |
Gene Name: | Histone H2A-Bbd type 1 |
Synonyms: | H2A Barr body-deficient, H2A.Bbd, Histone H2A-like 2, H2A.L.2, H2AL2, Histone H2Alike 2, Testis-specific expressed gene 1 protein, TSEG-1, H2ab1, H2afb1, H2al2a |
Immunogen: | Recombinant protein corresponding to Mouse H2ab1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 13 kDa |
Observed MW. | 13 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Mouse | 1: 1000-1: 2000 | testis |
IHC | Mouse | 1: 4000-1: 8800 | testis |
Background
Atypical histone H2A which replaces conventional H2A during late spermatogenesis and is involved in the replacement of histones to protamine in male germ cells . Core component of nucleosome: nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template . Nucleosomes containing H2AB1 only wrap 130 bp of DNA, compared to 147 bp for classical nucleosomes . In condensing spermatids, the heterodimer between H2AB1 and H2BC1/TH2B is loaded onto the nucleosomes and promotes loading of transition proteins (TNP1 and TNP2) onto the nucleosomes . Inclusion of the H2AB1-H2BC1/TH2B dimer into chromatin opens the nucleosomes, releasing the nucleosomal DNA ends and allowing the invasion of nucleosomes by transition proteins (TNP1 and TNP2) .
Images
 | Western blot analysis of H2ab1 (GB113146) at dilution of 1: 2000 |
 | Immunohistochemistry analysis of paraffin-embedded mouse testis using H2ab1 (GB113146) at dilution of 1: 8800 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
