Recombinant Anti-HA tag Rabbit mAb (100 μl)

Recombinant Anti-HA tag Rabbit mAb (100 μl)

Brand: ServiceBio
Cat. #: GB151253-100
Availability: In Stock
$259.00
-+
Reactivity:     
Applications: WB    
Host Species: Rabbit    
Clonality: Monoclonal    
Gene Name:

HA Tag

Synonyms:

HA epitope tag,HA1,HA2,hemagglutinin,HemagglutininHA1 chain,Hemagglutinin HA2 chain

Immunogen:

KLH conjugated Synthetic peptide corresponding to YPYDVPDYA

Isotype:

lgG

Purity:

Affinity purification

Subcellular location:

Mitochondrion, Cytoplasm




Product Usage Information

WB

Tag (All species)

1: 1000-1: 2000

HA-tag fusion protein




Background

Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The HA tag is corresponds to amino acid residues YPYDVPDYA of human influenza virus hemagglutinin(HA). Many recombinant proteins have been engineered to express the HA tag, which does not appear to interfere with the bioactivity or the biodistribution of the recombinant protein. This tag facilitates the detection, isolation, and purification of the proteins. The HA tag is useful in western blotting and immunohistochemical localization of expressed fusion proteins when examined with antibodies raised specifically against the HA-tag.




Images

01 GB151253_C496 重组 HA-Tag_WB.jpg

WB analysis of HA-Tag (GB151253).
Sample: Protein treated by RIPA Lysis Buffer (G2002).
Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h.
Primary antibody: 1: 1000, 4°C overnight.
Secondary antibody:  HRP Goat Anti-Rabbit lgG (GB23303), 1: 3000, RT, 1h.




Storage

Storage Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
Storage Buffer PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol.


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