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| Reactivity: | M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | HARS rabbit polyclonal antibody |
Gene Name: | Histidine--tRNA ligase, cytoplasmic |
Synonyms: | Histidyl-tRNA synthetase, FLJ20491, USH3B, HisRS, Hars1, Harshistidine translase, Histidine tRNA ligase |
Immunogen: | Recombinant protein corresponding to Mouse HARS |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 57 kDa |
Observed MW. | 57 kDa |
Uniprot ID: | Q61035 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 500-1: 1000 | liver, brain, lung |
Background
The gene is located in a head-to-head orientation with HARSL on chromosome five, where the homologous genes share a bidirectional promoter. Aminoacyl-tRNA synthetases are a class of enzymes that charge tRNAs with their cognate amino acids.Catalyzes the ATP-dependent ligation of histidine to the 3'-end of its cognate tRNA, via the formation of an aminoacyl-adenylate intermediate (His-AMP). Plays a role in axon guidance. The protein encoded by this gene is a cytoplasmic enzyme which belongs to the class II family of aminoacyl-tRNA synthetases. The enzyme is responsible for the synthesis of histidyl-transfer RNA, which is essential for the incorporation of histidine into proteins.
Images
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Western blot analysis of HARS (GB111576) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
