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Anti-HNF-4-alpha Rabbit pAb (100 μl)
| Reactivity: | H & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Hepatocyte nuclear factor 4-alpha |
Synonyms: | HNF-4-alpha, Nuclear receptor subfamily 2 group A member 1, Transcription factor 14, TCF-14, Transcription factor HNF-4, Hnf-4, Hnf4, Nr2a1, Tcf14, Hnf4a |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse HNF-4-alpha |
Uniprot ID: | P49698 |
Isotype: | lgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 53 kDa / 52 kDa |
Product Usage Information
WB | Human | 1: 300-1: 400 | HepG2 |
IHC/IF | Mouse, Rat | 1: 100-1: 200 | liver, kidney |
Background
Transcriptional regulator which controls the expression of hepatic genes during the transition of endodermal cells to hepatic progenitor cells, facilitatating the recruitment of RNA pol II to the promoters of target genes (By similarity). Activates the transcription of CYP2C38 Represses the CLOCK-ARNTL/BMAL1 transcriptional activity and is essential for circadian rhythm maintenance and period regulation in the liver and colon cells.
Images
| WB analysis of HNF4α (GB115549). |
| IHC analysis of HNF4 (GB115549). Sample: Mouse liver (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of HNF4 (GB115549). Sample: Rat liver (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
| IHC analysis of HNF4 (GB115549). Sample: Rat kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: TE buffer (pH9.0)(G1203), 98°C 20min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 100, 4°C overnight. Secondary antibody: HRP Goat Anti-Rabbit lgG (GB23303), 1: 200 RT 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
