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Recombinant Anti-HSP70 antibody (HRP Conjugated) (100 μl)
| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | |
| Clonality: | |
| Gene Name: | Heat shock 70 kDa protein 1A |
Synonyms: | HSP70-1, HSP70.1, HSPA1, HSP72, Heat Shock 70 KDa Protein 1 |
Immunogen: | Recombinant protein corresponding to Human HSP70 |
Uniprot ID: | P0DMV8 |
Isotype: | IgG1 |
Conjugation | HRP |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 70 kDa / 70 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
WB | Human, Mouse, Rat | 1: 500-1: 2000 | brain, HeLa, Hep G2 |
Incubation condition | After blocking, incubate the membrane with HRP conjugated Anti-HSP70 antibody(ZB15241-HRP) for 3-4 hours at room temperature (20-25°C) or overnight at 4°C, and then wash the membrane 3 times for 5 mins each with TBST. | ||
Background
This protein is a member of the heat shock protein 70 family. In conjuction with other heat shock proteins, this protein stabilizes existing proteins against aggregation and mediates the folding of newly translated proteins in the cytosol and in organelles. It is also involved in the ubiquitin-proteasome pathway through interaction with the AU-rich element RNA-binding protein 1. The gene is located in the major histocompatibility complex class III region, in a cluster with two closely related genes which encode similar proteins.
Images
| WB analysis of HSP70(ZB15241-HRP). Antibody: HRP conjugated anti-HSP70 antibody 1: 2000, 4°C overnight. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
