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Recombinant Anti-Heme Oxygenase 1 Mouse mAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Heme oxygenase 1 |
Synonyms: | HMOX1, HMOX1D, HO-1, HSP32, bK286B10, heme oxygenase 1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse HMOX1 |
Uniprot ID: | P14901 |
Isotype: | IgG1 |
Purity: | Affinity purification |
Subcellular location: | Endoplasmic reticulum membrane |
Predicted MW. / Observed MW. | 33 kDa / 33 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 300-1: 800 | spleen, liver, RAW264.7 |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | Inflammatory model induced by lps(24h) lung, Inflammatory model induced by lps(6h) spleen |
Background
Heme oxygenase cleaves the heme ring at the alpha methene bridge to form biliverdin. Biliverdin is subsequently converted to bilirubin by biliverdin reductase. Under physiological conditions, the activity of heme oxygenase is highest in the spleen, where senescent erythrocytes are sequestrated and destroyed. Exhibits cytoprotective effects since excess of free heme sensitizes cells to undergo apoptosis.
Images
| WB analysis of Heme Oxygenase 1(GB15104). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 3000, RT, 1h. |
| IHC analysis of Heme Oxygenase 1(GB15104). |
| IHC analysis of Heme Oxygenase 1(GB15104). |
| IHC analysis of Heme Oxygenase 1 (GB15104). Sample: Human colon cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
| IHC analysis of Heme Oxygenase 1 (GB15104). Sample: Human tonsil, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
| IHC analysis of Heme Oxygenase 1 (GB15104). Sample: liver cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Mouse lgG (H+L) (GB23301), 1: 200, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
