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Recombinant Anti-Hsp60 Mouse mAb (100 μl)
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | 60 kDa heat shock protein, mitochondrial |
Synonyms: | HSP60, HSP-60, HuCHA60, CPN60, 60 KDa Chaperonin, P60 Lymphocyte Protein, Heat Shock Protein 60 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse HSP60 |
Uniprot ID: | P63038 |
Isotype: | lgG1 |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion, Cytoplasm |
Predicted MW. / Observed MW. | 60 kDa / 60 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 500-1: 1000 | lung, brain |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 1000 | gastric carcinoma, kidney |
Background
Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. In addition to its role as a heat shock protein, HSP60 functions as a chaperonin to assist in folding linear amino acid chains into their respective three-dimensional structure.
Images
| WB analysis of HSP60 (GB15243). |
| IHC analysis of HSP60 (GB15243). Sample: Human gastric carcinoma (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h. |
| IHC analysis of HSP60 (GB15243). Sample: Mouse kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h. |
| IHC analysis of HSP60 (GB15243). Sample: Rat kidney (Paraffin), 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2min. Blocking buffer: 3% BSA in PBS (GC305010), RT, 30min. Primary antibody: 1: 500, 4°C overnight. Secondary antibody: HRP Goat Anti-Mouse lgG (GB23301), 1: 3000, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
