Your shopping cart is empty!
| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 60 kDa heat shock protein, mitochondrial |
Synonyms: | HSP60, HSP-60, HuCHA60, CPN60, 60 KDa Chaperonin, P60 Lymphocyte Protein, Heat Shock Protein 60 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse HSP60 |
Uniprot ID: | P63038 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Mitochondrion matrix |
Predicted MW. / Observed MW. | 60 kDa / 60 kDa |
Product Usage Information
WB | Human | 1: 3000-1: 10000 | spleen, brain |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 3000 | colon cancer, gastric cancer, kidney |
ICC/IF | Human, Mouse, Rat | 1: 100-1: 400 | NIH-3T3 |
IEM | Mouse | 1: 50-1: 100 | liver, heart, pancreas |
Background
Heat shock protein 60 (HSP60) is a mitochondrial chaperonin that is typically held responsible for the transportation and refolding of proteins from the cytoplasm into the mitochondrial matrix. In addition to its role as a heat shock protein, HSP60 functions as a chaperonin to assist in folding linear amino acid chains into their respective three-dimensional structure. Through the extensive study of groEL, HSP60’s bacterial homolog, HSP60 has been deemed essential in the synthesis and transportation of essential mitochondrial proteins from the cell's cytoplasm into the mitochondrial matrix.
Images
|
|
Western blot analysis of HSP60 (GB11243) at dilution of 1: 3000 |
|
Immunohistochemistry of paraffin embedded human colon cancer using HSP60 (GB11243) at dilution of 1: 1500 (200x lens) |
| Immunohistochemistry of paraffin embedded human colon cancer using HSP60 (GB11243) at dilution of 1: 1500 (300x lens) |
|
|
Immunohistochemistry of paraffin embedded mouse kidney using HSP60 (GB11243) at dilution of 1: 1500 (300x lens) |
|
Immunohistochemistry of paraffin embedded rat kidney using HSP60 (GB11243) at dilution of 1: 1500 (300x lens) |
|
|
Immunohistochemistry of paraffin embedded rat liver using HSP60 (GB11243) at dilution of 1: 1500 (300x lens) |
| Immunofluorescence of paraffin embedded human gastric cancer using HSP60 (GB11243) at dilution of 1: 1500 (300x lens) |
| Immunofluorescence of paraffin embedded rat ovary using HSP60 (GB11243) at dilution of 1: 1500 (300x lens) |
| Immunoelectron microscopy analysis of LR white resin-embedded mouse liver using HSP60 (GB11243) at dilution of 1: 50. A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50. |
 | Immunoelectron microscopy analysis of LR white resin-embedded mouse liver using HSP60 (GB11243) at dilution of 1: 50. A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50. |
 | Immunoelectron microscopy analysis of LR white resin-embedded mouse heart using HSP60 (GB11243) at dilution of 1: 50. A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50. |
 | Immunoelectron microscopy analysis of LR white resin-embedded mouse pancreas using HSP60 (GB11243) at dilution of 1: 50. A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50. |
 | Immunoelectron microscopy analysis of LR white resin-embedded mouse pancreas using HSP60 (GB11243) at dilution of 1: 50. A goat anti-rabbit antibody preabsorbed with 10nm colloidal gold was used as the secondary antibody, at dilution of 1: 50. |
| Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using Hsp60(GB11243) at dilution of 1:100-1:400 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
