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Anti-IL-2 Receptor alpha Rabbit pAb (100 μl)
| Reactivity: | M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | IL-2 Receptor alpha rabbit polyclonal antibody |
Gene Name: | Interleukin-2 receptor subunit alpha |
Synonyms: | IL2RA, CD25, IDDM10, IL2R, TCGFR, p55, IMD41, interleukin 2 receptor subunit alpha |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse IL2RA |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Membrane |
Predicted MW. | 31 kDa |
Observed MW. | 45 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 500-1: 1000 | splenitis, spleen, lymph node, thymus |
IHC/IF | Mouse, Rat | 1: 200-1: 1000 | liver, pneumonia, spleen |
Background
IL-2Rα(Activated T cells IL-2Rα Chain 55), also kown as membrane interleukin-2 receptor (mIL-2R) or CD25, is a protein encoded by IL2RA gene in Human. This protein is 55 kDa which is the high affinity composition of IL-2R. Few IL-2α chains will express in inactive T cells, while the expression will remarkably increase followed by IL-2 accumulation, when T cell surface receptor (TCR) is active. IL-2αchains will become soluble interleukin-2 receptor (sIL-2R) when they are detached from cell membrane into serum, and the molecular weight of sIL-2R is 45 kDa. The concentration of sIL-2R in serum can be used for a mark to measure T cells activation and IL-2Rα chains expression.
Images
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Western blot analysis of CD25 (GB11612) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded mouse liver using IL-2 Receptor alpha (GB11612) at dilution of 1: 200 (400x lens) |
 | Immunohistochemistry of paraffin embedded mouse pneumonia using IL-2 Receptor alpha (GB11612) at dilution of 1: 500 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse spleen using IL-2 Receptor alpha (GB11612) at dilution of 1: 200 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
