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| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | IST1 homolog |
Synonyms: | hIST1, IST1 homolog, KIAA0174, OLC1 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse IST1 |
Uniprot ID: | Q9CX00 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 39 kDa / 42 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 300-1: 600 | spleen, lung |
Background
ESCRT-III-like protein involved in specific functions of the ESCRT machinery. Is required for efficient abscission during cytokinesis, but not for HIV-1 budding. The involvement in the MVB pathway is not established. Involved in recruiting VPS4A and/or VPS4B to the midbody of dividing cells. During late anaphase, involved in nuclear envelope reassembly and mitotic spindle disassembly together with the ESCRT-III complex: IST1 acts by mediating the recruitment of SPAST to the nuclear membrane, leading to microtubule severing. Regulates early endosomal tubulation together with the ESCRT-III complex by mediating the recruitment of SPAST.
Images
| Western blot analysis of IST1 (GB115082) at dilution of 1: 600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
