Reactivity: | H,M & H,M,R |
Applications: | WB & IHC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Full Name: | HAT1 rabbit polyclonal antibody |
Gene Name: | Histone acetyltransferase type B catalytic subunit |
Synonyms: | Cysteine-S-conjugate beta-lyase, Glutamine transaminase K, GTK, Kyat1, Ccbl1, KATI, Glutamine--phenylpyruvate transaminase, Kynurenine aminotransferase 1, Kat, HAT1 |
Immunogen: | Recombinant protein corresponding to Mouse HAT1 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. | 48 kDa |
Observed MW. | 48 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Human, Mouse | 1: 500-1: 1000 | thymus |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 2000 | colon cancer, testis |
Background
Histone acetyltransferase 1, also known as HAT1, is an enzyme encoded by the HAT1 gene. The protein is a type B histone acetyltransferase (HAT) that is involved in the rapid acetylation of newly synthesized cytoplasmic histones, which are, in turn, imported into the nucleus for de novo deposition onto nascent DNA chains. Histone acetylation, in particular, of histone H4, plays an important role in replication-dependent chromatin assembly. To be specific, this HAT can acetylate soluble but not nucleosomal histone H4 at lysines 5 and 12, and, to a lesser degree, histone H2A at lysine 5.
Images
Western blot analysis of hat1 (GB11906) at dilution of 1: 500 |
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Immunohistochemistry of paraffin embedded human colon cancer using hat1 (GB11906) at dilution of 1: 2000 (250x lens) |
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Immunohistochemistry of paraffin embedded rat testis using hat1 (GB11906) at dilution of 1: 2000 (250x lens) |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |