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Anti-KLHL10 Rabbit pAb (100 μl)
| Reactivity: | M,R & M |
| Applications: | WB & ICC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | Kelch-like protein 10 |
Synonyms: | kelch like 10 (Drosophila), Kelch like protein 10, KLHL10 |
Immunogen: | Recombinant protein corresponding to Mouse KLHL10 |
Uniprot ID: | Q9D5V2 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 69 kDa / 65 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 1000-1: 2000 | heart |
ICC/IF | Mouse | 1: 50-1: 200 | NIH-3T3 |
Background
May be a substrate-specific adapter of a CUL3-based E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins during spermatogenesis. Required for male fertility.
Images
| WB analysis of KLHL10(GB114500). Sample: Protein treated by RIPA Lysis Buffer (G2002). Blocking buffer: 3% Nonfat dry milk (GC310001) in TBST, RT, 1h. Primary antibody: 1: 1000, 4°C overnight. Secondary antibody: HRP conjugated Goat Anti-Rabbit lgG (H+L) (GB23303), 1: 3000, RT, 1h. |
| ICC analysis of KLHL10(GB114500)red. Sample: NIH-3T3, 4% PFA (G1101) 20min. Blocking buffer: 3% BSA (GC305010) in PBS, RT, 30min. Primary antibody: 1: 200, 4°C overnight. Secondary antibody: Cy3 conjugated Goat Anti-Rabbit IgG (H+L) (GB21303), 1: 300, RT, 1h. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
