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| Reactivity: | M,R & H,M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | LAT rabbit polyclonal antibody |
Gene Name: | Linker for activation of T-cells family member 1 |
Synonyms: | 36 kDa phospho-tyrosine adapter protein, pp36, p36-38, Lat |
Immunogen: | Recombinant protein corresponding to Mouse LAT |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Cell membrane |
Predicted MW. | 26 kDa |
Observed MW. | 38 kDa |
Uniprot ID: | O54957, O70601 |
Product Usage Information
Applications | Species | Dilution | Positive Sample |
WB | Mouse, Rat | 1: 500-1: 1000 | spleen, thymus, lymph |
IHC/IF | Human, Mouse, Rat | 1: 500-1: 4000 | colon, thymus |
Background
Linker for activation of T-cells family member 1 (LAT) is a single-pass type III membrane protein which is required for TCR (T-cell antigen receptor) and pre-TCR-mediated signaling, both in mature T-cells and during their development This protein is involved in FCGR3 (low affinity immunoglobulin gamma Fc region receptor III)-mediated signaling in natural killer cells and FCER1 (high affinity immunoglobulin epsilon receptor)-mediated signaling in mast cells. Functionally T cell receptor (TCR) activation results in tyrosine phosphorylation of LAT, leading to the direct interaction with several proteins, including PLC-gamma 1, Grb2 and Gads.
Images
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Western blot analysis of Lat (GB111123) at dilution of 1: 500 |
 | Immunohistochemistry of paraffin embedded mouse thymus using Lat (GB111123) at dilution of 1: 2000 (400x lens) |
 | Immunohistochemistry of paraffin embedded rat thymus using Lat (GB111123) at dilution of 1: 2000 (400x lens) |
 | Immunofluorescence of paraffin embedded human colon using Lat (GB111123) at dilution of 1: 2100 (400x lens) |
 | Immunofluorescence of paraffin embedded mouse thymus using Lat (GB111123) at dilution of 1: 2100 (400x lens) |
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Immunofluorescence of paraffin embedded rat colon using Lat (GB111123) at dilution of 1: 2100 (400x lens) |
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Immunofluorescence of paraffin embedded rat thymus using Lat (GB111123) at dilution of 1: 2100 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
