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| Reactivity: | H,M,R & M,R |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | DNA replication licensing factor MCM2 |
Synonyms: | Minichromosome maintenance protein 2 homolog, Nuclear protein BM28, Mcm2, Bm28, Cdcl1, Kiaa0030, Mcmd2 |
Immunogen: | Recombinant protein corresponding to Mouse MCM2 |
Uniprot ID: | P97310 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 102 kDa / 130 kDa |
Applications
WB | Human, Mouse, Rat | 1: 500-1: 1000 | thymus, lymph node |
IHC/IF | Mouse, Rat | 1: 500-1: 3600 | small intestine, colon, kidney |
Background
The MCM2-7 complex forms the core of the replicative helicase which acts as the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork in eukaryotes. This complex is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM2, also named CDCL1 and BM28, is a human nuclear protein that plays an important role in 2 crucial steps of the cell cycle, namely, onset of DNA replication and cell division.
Images
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Western blot analysis of MCM2 (GB111361) at dilution of 1: 1000 |
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Immunohistochemistry analysis of paraffin embedded rat colon using MCM2 (GB111361) at dilution of 1: 950 |
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Immunohistochemistry analysis of paraffin embedded mouse small intestine using MCM2 (GB111361) at dilution of 1: 950 |
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Immunofluorescent analysis of paraffin embedded mouse lymph using MCM2 (GB111361) at dilution of 1: 1900 |
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Immunofluorescent analysis of paraffin embedded mouse kidney using MCM2 (GB111361) at dilution of 1: 1900 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
