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| Reactivity: | H,M,R & H,M,R |
| Applications: | WB & IHC |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | MCM6 rabbit polyclonal |
Gene Name: | DNA replication licensing factor MCM6 |
Synonyms: | Mis5 homolog, Mcm6, Mcmd6, Mis5, P105MCM |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse MCM6 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | |
Predicted MW. | 93 kDa |
Observed MW. | 105 kDa |
Uniprot ID: |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 1000-1: 2000 | spleen |
IHC | Human, Mouse, Rat | 1: 1800-1: 3600 | tonsil, spleen |
Background
The MCM genes were firstly identified in yeast defective in minichromosome maintenance and have since been shown to have roles in the progression of the cell cycle, and most of them are cell division control genes. MCM2-7 complex are suggested to be 'DNA licensing factors' which bind to the DNA after mitosis and enable DNA replication before being removed during S phase. Mini-chromosome maintenance 6 (MCM6) is one component of the MCM2-7 complex which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. MCM6 single subunit forms homohexamer and contains an ATP-dependent and replication fork stimulated 3' to 5' DNA unwinding activity along with intrinsic DNA-dependent ATPase and ATP-binding activities .
Images
| Western blot analysis of MCM6 (GB112993) at dilution of 1: 2000 Lane 1: HeLa cell lysate Lane 2: Jurkat cell lysate Lane 3: C6 cell lysate Lane 4: RAW264.7 cell lysate Lane 5: PC-12 cell lysate Lane 6: Mouse spleen tissue lysate |
| Immunohistochemistry analysis of paraffin-embedded human tonsil using MCM6 (GB112993) at dilution of 1: 3600 |
| Immunohistochemistry analysis of paraffin-embedded mouse spleen using MCM6 (GB112993) at dilution of 1: 3600 |
| Immunohistochemistry analysis of paraffin-embedded rat spleen using MCM6 (GB112993) at dilution of 1: 3600 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
