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| Reactivity: | M,R |
| Applications: | IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | MDM2 rabbit polyclonal antibody |
Gene Name: | E3 ubiquitin-protein ligase Mdm2 |
Synonyms: | Double minute 2 protein, Oncoprotein Mdm2, RING-type E3 ubiquitin transferase Mdm2, p53-binding protein Mdm2 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse MDM2 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus, Cytoplasm |
Uniprot ID: | P23804 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
IHC | Mouse, Rat | 1: 1000-1: 3000 | kidney, lung, thymus |
Background
MDM2 is a target of the transcription factor tumor protein p53. The encoded protein is a nuclear phosphoprotein that binds and inhibits transactivation by tumor protein p53, as part of an autoregulatory negative feedback loop. Over expression of MDM2 can result in excessive inactivation of tumor protein p53, diminishing its tumor suppressor function. This protein has E3 ubiquitin ligase activity, which targets tumor protein p53 for proteasomal degradation. This protein also affects the cell cycle,apoptosis, and tumorigenesis through interactions with other proteins, including retinoblastoma 1 and ribosomal protein L5.
Images
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Immunohistochemistry of paraffin embedded mouse kidney using MDM2 (GB111161) at dilution of 1:1500 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse lung using MDM2 (GB111161) at dilution of 1:1500 (400x lens) |
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Immunohistochemistry of paraffin embedded mouse thymus using MDM2 (GB111161) at dilution of 1:1500 (400x lens) |
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Immunohistochemistry of paraffin embedded rat kidney using MDM2 (GB111161) at dilution of 1:1500 (400x lens) |
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Immunohistochemistry of paraffin embedded rat lung using MDM2 (GB111161) at dilution of 1:1500 (400x lens) |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
