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Anti-MEPCE Rabbit pAb (100 μl)
| Reactivity: | H,M,R & M |
| Applications: | WB & IHC/IF |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Gene Name: | 7SK snRNA methylphosphate capping enzyme |
Synonyms: | Bin3 homolog, MEPCE, methylphosphate capping enzyme |
Immunogen: | Recombinant protein corresponding to Mouse MEPCE |
Uniprot ID: | Q8K3A9 |
Isotype: | IgG |
Purity: | Affinity purification |
Subcellular location: | Nucleus |
Predicted MW. / Observed MW. | 72 kDa / 69 kDa |
Product Usage Information
WB | Human, Mouse, Rat | 1: 800-1: 1500 | liver |
IHC/IF | Mouse | 1: 2000-1: 4000 | testis |
Background
S-adenosyl-L-methionine-dependent methyltransferase that adds a methylphosphate cap at the 5'-end of 7SK snRNA (7SK RNA), leading to stabilize it (By similarity). Also has a non-enzymatic function as part of the 7SK RNP complex: the 7SK RNP complex sequesters the positive transcription elongation factor b (P-TEFb) in a large inactive 7SK RNP complex preventing RNA polymerase II phosphorylation and subsequent transcriptional elongation . The 7SK RNP complex also promotes snRNA gene transcription by RNA polymerase II via interaction with the little elongation complex (LEC) (By similarity). In the 7SK RNP complex, MEPCE is required to stabilize 7SK RNA and facilitate the assembly of 7SK RNP complex (By similarity). MEPCE has a non-enzymatic function in the 7SK RNP complex; it has a non-enzymatic function; interaction with LARP7 within the 7SK RNP complex occluding its catalytic center (By similarity).
Images
| Western blot analysis of MEPCE (GB114256) at dilution of 1: 1000 |
| Immunohistochemistry analysis of paraffin embedded mouse testis using MEPCE (GB114256) at dilution of 1: 3000 |
| Immunofluorescence analysis of paraffin embedded mouse testis using MEPCE (GB114256) at dilution of 1: 2000 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |
