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| Reactivity: | H |
| Applications: | IHC/IF |
| Host Species: | Mouse |
| Clonality: | Monoclonal |
| Gene Name: | Matrix metalloproteinase-9 |
Synonyms: | MMP9, CLG4B, GELB, MANDP2, MMP-9, 92 kDa type IV collagenase, 92 kDa gelatinase, gelatinase B, matrix metallopeptidase 9 |
Immunogen: | Recombinant protein corresponding to Human MMP9 |
Uniprot ID: | P14780 |
Isotype: | IgG2a,k |
Purity: | Affinity purification |
Subcellular location: | Secreted |
Predicted MW. / Observed MW. | 78 kDa / 92 kDa |
Product Usage Information
Applications | Species | Dilution | Positive Tissue |
IHC/IF | Human | 1: 300-1: 600 | tonsil, esophagus, lung |
Background
May play an essential role in local proteolysis of the extracellular matrix and in leukocyte migration. Could play a role in bone osteoclastic resorption. Cleaves KiSS1 at a Gly-|-Leu bond. Cleaves type IV and type V collagen into large C-terminal three quarter fragments and shorter N-terminal one quarter fragments. Degrades fibronectin but not laminin or Pz-peptide.
Images
| IHC analysis of MMP9(GB120004). Sample: Human tonsil, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L),Ready to use(G1301), RT, 20min. |
| IHC analysis of MMP9(GB120004). Sample: Human esophagus, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L),Ready to use(G1301), RT, 20min. |
| IHC analysis of MMP9(GB120004). Sample: Human liver, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L),Ready to use(G1301), RT, 20min. |
| IHC analysis of MMP9(GB120004). Sample: Human lung cancer, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L),Ready to use(G1301), RT, 20min. |
| IHC analysis of MMP9(GB120004). Sample: Human lung, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L),Ready to use(G1301), RT, 20min. |
| IHC analysis of MMP9(GB120004). Sample: Human stomach, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L),Ready to use(G1301), RT, 20min. |
| IHC analysis of MMP9(GB120004). Sample: Human tonsil, 4% PFA (G1101) 12-24h. Antigen retrieval: Citrate buffer (pH 6.0) (G1201), pressure cooker 2 min. Primary antibody: 1: 300, 4°C overnight. Secondary antibody: S-vision poly-HRP conjugated Goat Anti-Mouse lgG(H+L),Ready to use(G1301), RT, 20min. |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.15% ProClin300, 100 μg/mL BSA and 50% glycerol. |
