Reactivity: | H & H,M |
Applications: | WB & ICC/IF |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | 39S ribosomal protein L44, mitochondrial |
Synonyms: | L44mt, MRP-L44, Mitochondrial large ribosomal subunit protein mL44, RPL44 |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Human MRPL44 |
Uniprot ID: | Q9H9J2 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 38 kDa / 38 kDa |
Images
WB | Human | 1: 500-1: 1000 | HeLa, 293T, CaCO2 |
ICC/IF | Human, Mouse | 1: 50-1: 200 | HeLa, NIH-3T3 |
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. MRP-L44 (mitochondrial ribosomal protein L44) is a 332 amino acid protein that localizes to the mitochondrion, where it exists as a component of the 39S ribosomal subunit and works in conjunction with other MRPs to mediate protein synthesis.
Images
Western blot analysis of MRPL44 (GB111445) at dilution of 1: 500 |
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Immunocytochemistry analysis of 4% paraformaldehyde-fixed HeLa cells using MRPL44(GB111445) at dilution of 1: 50-1: 200 | |
Immunocytochemistry analysis of 4% paraformaldehyde-fixed NIH-3T3 cells using MRPL44(GB111445) at dilution of 1: 50-1: 200 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |