Reactivity: | M,R |
Applications: | WB |
Host Species: | Rabbit |
Clonality: | Polyclonal |
Gene Name: | 28S ribosomal protein S31, mitochondrial |
Synonyms: | Imogen 38, IMOGN38, MRP S31, S31mt |
Immunogen: | KLH conjugated Synthetic peptide corresponding to Mouse MRPS31 |
Uniprot ID: | Q61733 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. / Observed MW. | 44 kDa / 37 kDa |
Product Usage Information
WB | Mouse, Rat | 1: 3000-1: 4000 | liver |
Background
Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology.
Images
Western blot analysis of MRPS31 (GB115006) at dilution of 1: 3000 |
Storage
Storage | Store at -20°C for one year. Avoid repeated freeze/thaw cycles. |
Storage Buffer | PBS with 0.02%sodium azide,100 μg/ml BSA and 50% glycerol. |