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| Reactivity: | H,M,R |
| Applications: | WB |
| Host Species: | Rabbit |
| Clonality: | Polyclonal |
| Full Name: | MSK1 rabbit polyclonal antibody |
Gene Name: | Ribosomal protein S6 kinase alpha-5 |
Synonyms: | Rps6ka5, RPS6KA5, MSK1, MSPK1, RLPK, Ribosomal protein S6 kinase A5, Mitogen and stress activated protein kinase 1 |
Immunogen: | Recombinant protein corresponding to Mouse MSK1 |
Isotype: | IgG |
Purity: | Affinity purification |
Predicted MW. | 90 kDa |
Observed MW. | 90 kDa |
Uniprot ID: | O75582, Q8C050 |
Product Usage Information
Applications | Species | Dilution | Positive tissue |
WB | Human, Mouse, Rat | 1: 500-1: 1000 | brain, lung, thymus |
Background
Ribosomal protein S6 kinase alpha-5 is an enzyme encoded by the RPS6KA5 gene. MSK1 is a mitogen and stress activated protein kinase 1 which belongs to the AGC family of kinases and is related in structure to the ribosomal p70 S6 kinase subfamily. MSK1 can be activated by ERK1/2 and SAPK2/p38 MAP kinase. It is also known to be required for the phosphorylation of CREB, ATF1 H3 and HMG14 in response to mitogen and stress. Similar to RSK, MSK1 contains two kinase domains (N term and a C term). Once phosphorylated on Thr581 and Ser360 by ERK1/2 and SAPK2/p38, MSK1 autophosphorylate on at least 5 sites. Of these autophosphorylation sites Ser212 and Ser376 get phosphorylated by the C terminal kinase domain of MSK1 which is essential for the catalytic activity of the N terminal kinase domain.
Images
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Western blot analysis of RLSK (GB11857) at dilution of 1: 500 |
Storage
| Storage | Store at -20°C for one year. Avoid repeated freeze/ thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 100 μg/ml BSA and 50% glycerol. |
